The purpose of this study was to characterize experimental n-heptane combustion behavior in a direct-injection constant-volume combustion chamber (DI-CVCC), using chamber pressure to infer ignition delay and heat-release rate. Measurements generally displayed expected trends and indicated entirely premixed combustion with no mixing-controlled phase. A significant finding was the observation of negative temperature coefficient (NTC) behavior. Comparing results with CHEMKIN-PRO simulations, it was found that a homogeneous combustion model was reasonably accurate for ignition delays longer than 5 ms. The combination of NTC behavior and homogeneous fuel-air mixtures suggests that this DI-CVCC can be useful for validation of chemical-kinetic mechanisms.