The purpose of this study was to determine the kinetics of HSP70 expression in response to mild thermal stress. The rationale is to produce a basis for design of optimal heating methods to induce HSP70 expression for preconditioning in cardiac surgery. Bovine aortic endothelial cells were heated at 42°C for 0.5 to 5 hours followed by 37°C recovery for 1 to 48 hours. Quantitative analysis of western blot results showed HSP70 expression kinetics is a coupled function of heating temperature and time and of post-heating duration. Bimodal HSP70 expression kinetics were identified which may be an important cause of the “second window of protection” observed by other researchers.

Issue Section:
Technical Papers
Keywords:
proteins, biothermics, cardiology, surgery, cellular biophysics
Topics:
Heat, Heating, Proteins, Shock (Mechanics), Temperature, Surgery, Thermal stresses, Stress
1.
Kiang
,
J. G.
, and
Tsokos
,
G. C.
,
1998
, “
Heat Shock Protein 70kDa: Molecular Biology, Biochemistry, and Physiology
,”
Pharmacol. Ther.
,
80-2
, pp.
183
–
201
.
2.
Ritossa
,
F.
,
1962
, “
A New Puffing Pattern Induced by Temperature Shock and DNP in Drosophila
,”
Experientia
,
18
, pp.
571
–
573
.
3.
Gray
,
C. C.
,
Amrani
,
M.
, and
Yacoub
,
M. H.
,
1999
, “
Heat Stress Proteins and Myocardial Protection: Experimental Model or Potential Clinical Tool?
”
Int. J. Biochem. Cell Biol.
,
31
, pp.
559
–
573
.
4.
Yenari
,
M. A.
,
Giffard
,
R. G.
,
Sapolsky
,
R. M.
, and
Steinberg
,
G. K.
,
1999
, “
The Neuroprotective Potential of Heat Shock Protein 70 (HSP70)
,”
Mol. Med. Today
,
5
, pp.
525
–
531
.
5.
Beck
,
F.
,
Neuhofer
,
W.
, and
Muller
,
E.
,
2000
, “
Molecular Chaperones in the Kidney: Distribution, Putative Roles, and Regulation
,”
Am. J. Physiol.
,
279
, pp.
F203–F215
F203–F215
.
6.
Tsuruma
,
T.
,
Yagihashi
,
A.
,
Matsuno
,
T.
,
Zou
,
X. M.
,
Asanuma
,
K.
,
Sasaki
,
K.
, and
Hirata
,
K.
,
1996
, “
The Heat Shock Protein 70 Family Reduces Ischemia-reperfusion Injury in Small Intestine
,”
Transplant. Proc.
,
28
, pp.
2629
–
2630
.
7.
Rokutan
,
K.
,
2000
, “
Gastric Mucosal Protection and Cell Proliferation: Role of Heat Shock Proteins in Gastric Mucosal Protection
,”
J. Gastroenterol. Hepatol
,
15
(
Suppl
), pp.
D12–D19
D12–D19
.
8.
Luft
,
J. C.
, and
Dix
,
D. J.
,
1999
, “
Hsp70 Expression and Function During Embryogenesis
,”
Cell Stress & Chaperones
,
4-3
, pp.
162
–
170
.
9.
Morimoto
,
R. I.
, and
Santoro
,
M. G.
,
1998
, “
Stress-inducible Responses and Heat Shock Proteins: New Pharmacologic Targets for Cytoprotection
,”
Nat. Biotechnol.
,
16
,
September
, pp.
833
–
838
.
10.
Currie
,
R. W.
,
Karmazyn
,
M.
,
Kloc
,
M.
, and
Mailer
,
K.
,
1988
, “
Heat Shock Response is Associated with Enhanced Postischemic Ventricular Recovery
,”
Circ. Res.
,
63
, pp.
543
–
549
.
11.
Gowda
,
A.
,
Yang
,
C. J.
,
Asimakis
,
G. K.
,
Ruef
,
J.
,
Rastegar
,
S.
,
Runge
,
M. S.
, and
Motamedi
,
M.
,
1998
, “
Cardioprotection by Local Heating: Improved Myocardial Salvage after Ischemia and Reperfusion
,”
Arch. Androl
,
65
, pp.
1241
–
1247
.
12.
Donnelly
,
T. J.
,
Sievers
,
R. E.
,
Vissern
,
F. L.
,
Welch
,
W. J.
, and
Wolfe
,
C. L.
,
1992
, “
Heat Shock Protein Induction in Rat Hearts: A Role for Improved Myocardial Salvage after Ischemia and Reperfusion?
”
Circulation
,
85
, pp.
769
–
778
.
13.
Amrani
,
M.
,
Latif
,
N.
,
Morrison
,
K.
,
Gray
,
C. C.
,
Jayakumar
,
J.
,
Corbett
,
J.
,
Goodwin
,
A. T.
,
Dunn
,
M. J.
, and
Yacoub
,
M. H.
,
1997
, “
Relative Induction of Heat Shock Protein in Coronary Endothelial Cells and Cardiomyocytes: Implications for Myocardial Protection
,”
J. Thorac. Cardiovasc. Surg.
,
115
, pp.
200
–
209
.
14.
Yellon
,
D. M.
,
Baxter
,
G. F.
,
1995
, “
A ‘Second Window of Protection’ or Delayed Preconditioning Phenomenon: Future Horizons for Myocardial Protection?
”
J. Cell. Cardiol.
,
27-4
, pp.
1023
–
1034
.
15.
Pagliaro
,
P.
,
Gattullo
,
D.
,
Rastaldo
,
R.
, and
Losano
,
G.
,
2001
, “
Ischemic Preconditioning: from the First to the Second Window of Protection
,”
Life Sci.
,
69
, pp.
1
–
15
.
16.
Karmazyn
,
M.
,
Mailer
,
K.
, and
Currie
,
R. W.
,
1990
, “
Acquisition and Decay of Heat-Shock-Enhanced Postischemic Ventricular Recovery
,”
Am. J. Physiol. Heart Circ. Physiol.
,
259
, pp.
H424–H431
H424–H431
.
17.
Cornelussen
,
R. N.
,
Garnier
,
A. V.
,
van der Vusse
,
G. J.
,
Reneman
,
R. S.
, and
Snoeckx
,
L. H. E. H.
,
1998
, “
Biphasic Effect of Heat Stress Pretreatment on Ischemic Tolerance of Isolated Rat Hearts
,”
J. Mol. Cell. Cardiol.
,
30
, pp.
365
–
372
.
18.
Hutter
,
M. M.
,
Sievers
,
R. E.
,
Barbosa
,
V.
, and
Wolfe
,
C. L.
,
1994
, “
Heat-Shock Protein Induction in Rat Hearts: a Direct Correlation between the Amount of Heat-Shock Protein Induced and the Degree of Myocardial Protection
,”
Circulation
,
89-1
, pp.
355
–
360
.
Copyright © 2003
 by ASME
You do not currently have access to this content.