The ion diffusion in an open nanofluidic system is studied by using an array of nanochannels. The mechanism of the ion diffusion was described through electrowetting-based nanofluidics. An ion diffusion experiment was conducted to validate the theoretical study of the relationship between the diffusion length and the ionic concentration using sodium chloride and phosphate buffer solutions. Shadow edge lithography was utilized to fabricate an array of open nanochannels, which allowed for a direct observation of the molecular diffusion through optical microscopy. The open channel configuration was then applied to stretching -DNA molecules in the nanochannels. The stretched length was measured by fluorescence microscopy. The presented nanofluidic device can be applied to the single-molecule study, which can benefit nanoengineered medicine and biology.
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e-mail: woonhong@uw.edu
e-mail: hoonlee@uw.edu
e-mail: qig210@lehigh.edu
e-mail: yal310@lehigh.edu
e-mail: youxia@uw.edu
e-mail: jstam@uw.edu
e-mail: jae71@uw.edu
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February 2011
Research Papers
Ion Diffusion and DNA Stretching in an Open Nanofluidic System
Woon-Hong Yeo,
Woon-Hong Yeo
Department of Mechanical Engineering,
e-mail: woonhong@uw.edu
University of Washington
, P.O. Box 352600, Seattle, WA 98195
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Kyong-Hoon Lee,
Kyong-Hoon Lee
Department of Mechanical Engineering,
e-mail: hoonlee@uw.edu
University of Washington
, P.O. Box 352600, Seattle, WA 98195
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Qingjiang Guo,
Qingjiang Guo
Department of Mechanical Engineering and Mechanics,
e-mail: qig210@lehigh.edu
Lehigh University
, Bethlehem, PA 18015
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Yaling Liu,
Yaling Liu
Department of Mechanical Engineering and Mechanics,
e-mail: yal310@lehigh.edu
Lehigh University
, Bethlehem, PA 18015
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Xia You,
Xia You
Department of Genome Sciences,
e-mail: youxia@uw.edu
University of Washington
, P.O. Box 355065, Seattle, WA 98195
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John A. Stamatoyannopoulos,
John A. Stamatoyannopoulos
Department of Genome Sciences,
e-mail: jstam@uw.edu
University of Washington
, P.O. Box 355065, Seattle, WA 98195
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Jae-Hyun Chung
Jae-Hyun Chung
Department of Mechanical Engineering,
e-mail: jae71@uw.edu
University of Washington
, Box 352600, Seattle, WA 98195
Search for other works by this author on:
Woon-Hong Yeo
Department of Mechanical Engineering,
University of Washington
, P.O. Box 352600, Seattle, WA 98195e-mail: woonhong@uw.edu
Kyong-Hoon Lee
Department of Mechanical Engineering,
University of Washington
, P.O. Box 352600, Seattle, WA 98195e-mail: hoonlee@uw.edu
Qingjiang Guo
Department of Mechanical Engineering and Mechanics,
Lehigh University
, Bethlehem, PA 18015e-mail: qig210@lehigh.edu
Yaling Liu
Department of Mechanical Engineering and Mechanics,
Lehigh University
, Bethlehem, PA 18015e-mail: yal310@lehigh.edu
Xia You
Department of Genome Sciences,
University of Washington
, P.O. Box 355065, Seattle, WA 98195e-mail: youxia@uw.edu
John A. Stamatoyannopoulos
Department of Genome Sciences,
University of Washington
, P.O. Box 355065, Seattle, WA 98195e-mail: jstam@uw.edu
Jae-Hyun Chung
Department of Mechanical Engineering,
University of Washington
, Box 352600, Seattle, WA 98195e-mail: jae71@uw.edu
J. Nanotechnol. Eng. Med. Feb 2011, 2(1): 011004 (6 pages)
Published Online: January 4, 2011
Article history
Received:
October 11, 2010
Revised:
November 10, 2010
Online:
January 4, 2011
Published:
January 4, 2011
Citation
Yeo, W., Lee, K., Guo, Q., Liu, Y., You, X., Stamatoyannopoulos, J. A., and Chung, J. (January 4, 2011). "Ion Diffusion and DNA Stretching in an Open Nanofluidic System." ASME. J. Nanotechnol. Eng. Med. February 2011; 2(1): 011004. https://doi.org/10.1115/1.4003029
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